Technical Skills
Molecular Biology
CRISPR + lsDNA (long single stranded DNA)
I used CRISPR to generate conditional knockout mice model. My strategy included long single stranded DNA (3kb).
- Various cloning methods (e.g. Hifi Assembly, Gateway, traditional)
- Real-Time PCR
- Various PCR methods
- Cryosection
- Immunohistochemistry
- Capillary electrophoresis
Animal related / Microscopy
- Mouse studies including embryonic stages (FELASA function A certification)
- Microinjection
- Zebrafish maintenance
Bioinformatics / NGS
- mRNA library prep. QC analysis with Fragment analyzer/Qubit
- Basic BASH and intermediate level R
- Bioconductor packages
- can analyze and interpret bulk RNAseq data
- Seurat (single cell)
Protein related
- Protein extraction from various materials
- Protein purification
- Western blot
Cell culture
- Generation of stable cell lines
- Grew MDCK on filters to study the effect of cell polarity
- Primary cell culture
- Cell maintenance (I worked with more than 4 different cell line)
- preparation of mouse embryonic fibroblast
- Protein, DNA ,RNA extraction from cells
Microscopy
- Confocal microscopy (Z-stacks)
- Live cell imaging
Soft Skills
Good communication
-Lived in different countries, worked in a global teams and communicated successfully with people from different background
Effective time and project management
-Successfully managed and perform multiple projects and delivered in time. Established cooperation with other research
groups (both natioanally and internationally) that resulted in one of my key research outcome.
Critical thinking and problem solving
I assisted
in their projects.
Money awareness, decision making and risk management
-As market conditions change rapidly, one must adapt to the changes and take quick actions to prevent loss. By trading, I am able to simulate these conditions and consequently increased my financial literacy and improved risk & budget management skills. I apply these skills to every other field in the life.